Journal: Molecular Neurobiology

Article Title: Melatonin Reduces Neuroinflammation and Improves Axonal Hypomyelination by Modulating M1/M2 Microglia Polarization via JAK2-STAT3-Telomerase Pathway in Postnatal Rats Exposed to Lipopolysaccharide

doi: 10.1007/s12035-021-02568-7

Figure Lengend Snippet: Primary antibodies used in experiments

Article Snippet: MT1 , Rabbit , Bioss , bs-0027R , WB (1:1000)/IF (1:100).

Techniques: Concentration Assay

Journal: Frontiers in Pharmacology

Article Title: An Improved Genetically Encoded Fluorescent cAMP Indicator for Sensitive cAMP Imaging and Fast Drug Screening

doi: 10.3389/fphar.2022.902290

Figure Lengend Snippet: Adipose tissue extract activates the orphan GPCR GPR50 in HEK293T cells. (A) Representative fluorescence images (left) and traces of ΔF/F 0 in response to 60 μM Fsk of G-Flamp2 in HEK239T cells stably expressing Empty Vector (EV) or GPR50 receptor (GPR50) (right). n = 14 and 10 cells for EV and GPR50, respectively. (B) Peak ΔF/F 0 in response to 60 μM Fsk in HEK293T cells stably expressing EV or GPR50. (C) Quantification of intracellular cAMP levels in EV and GPR50 cells after 20 min in stimulation of 60 μM n = 3 cultures for both. (D) Peak ΔF/F 0 in response to 5 mg/ml adipose extracts pretreatment for 5 min with 60 μM Fsk in HEK293T cells stably expressing EV or GPR50. Scale bars: 50 μm. Data are presented as the mean ± standard deviation (SD). * p < 0.05, *** p < 0.001 as measured by a Two-tailed Student’s t -test.

Article Snippet: Cells were then incubated with diluted primary antibody against GPR50 (#19762-AP, Proteintech, China) at 4°C overnight.

Techniques: Fluorescence, Stable Transfection, Expressing, Plasmid Preparation, Standard Deviation, Two Tailed Test

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model

doi: 10.1016/j.jcmgh.2022.07.007

Figure Lengend Snippet: List of Antibodies

Article Snippet: MT1 , IF , Rabbit polyclonal , Orb11085 , biorbyt (Cambridge, UK).

Techniques:

Journal: PLOS ONE

Article Title: Expression of circadian regulatory genes is dysregulated by increased cytokine production in mice subjected to concomitant intestinal injury and parenteral nutrition

doi: 10.1371/journal.pone.0290385

Figure Lengend Snippet: Antibodies used for Western analysis in this study.

Article Snippet: Mtnr1b , Novus , NLS932.

Techniques: Western Blot